A549细胞

A549是人类非小细胞肺癌细胞系，在1972年由科学家将一个58岁白人男性的外植体肿瘤转移并培养而成的^[1]。有研究指出侧群细胞亚群约占A549细胞总数的5.93%^[2]。在肺组织发现的A549细胞呈鳞状，负责水和电解质等物质在整个肺泡中的扩散，又可以通过胞磷胆碱途径合成含有高度不饱和脂肪酸的卵磷脂。

科研用途

A549细胞具有快速的增生速度可归因于环氧合酶2的大量表达。如果将A549细胞进行体外培养，就会生长为附著或紧贴在培养基上的单层细胞^[1]。当生长足够长的时间时，A549细胞会进行细胞分化。A549细胞目前被应用作研究肺癌和开发针对其的药物疗法的模型^[3]。A549细胞已成为II型肺泡上皮细胞的模型，并且在研究肺组织的代谢过程及将药物输送到组织的可能机制中具有实用性^[4]。同时这些细胞已分别通过细胞培养和异种移植，在体外和体内作为紫杉醇、欧洲紫杉醇和贝伐单抗的试验地方，以开发新的肺癌药物。

对A549细胞的深入研究已能阐明细胞谱系树 (pedigree-tree) 的概况，并且证明姐妹细胞 (sister cells) 之间的行为相关性^[5]。这种观察可以作为一种测量工具，确定细胞应激及遗传是对药物治疗的反应^[6]。A549细胞也可应用于病毒研究和相关的蛋白质表达变化^[7]。尽管A549细胞是癌细胞系，但是科研人员也会研究它对结核病的反应，特别是趋化因子的产生，因为它是由入侵的细菌诱导的^[8]。

参考资料

^ ^1.0 ^1.1 A549 Cell Line: Human alveolar adenocarcinoma cell line -General Information. [2019-12-03]. （原始内容存档于2012-01-05）（英语）.
^ Xia, H; Yu, C; Zhang, W; Zhang, B; Zhao, Y; Fang, F. [Identification and isolation of cancer stem cells from A549 cells].. Zhongguo fei ai za zhi = Chinese journal of lung cancer. 2013-08-20, 16 (8): 400–404 [2019-12-03]. PMID 23945242. doi:10.3779/j.issn.1009-3419.2013.08.02.
^ Giard, DJ; Aaronson, SA; Todaro, GJ; Arnstein, P; Kersey, JH; Dosik, H; Parks, WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.. Journal of the National Cancer Institute. 1973-11, 51 (5): 1417–23 [2019-12-03]. PMID 4357758. doi:10.1093/jnci/51.5.1417.
^ Foster, KA; Oster, CG; Mayer, MM; Avery, ML; Audus, KL. Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism.. Experimental cell research. 1998-09-15, 243 (2): 359–66 [2019-12-03]. PMID 9743595. doi:10.1006/excr.1998.4172.
^ Korsnes, MS; Korsnes, R. Single-Cell Tracking of A549 Lung Cancer Cells Exposed to a Marine Toxin Reveals Correlations in Pedigree Tree Profiles.. Frontiers in oncology. 2018, 8: 260 [2019-12-26]. PMID 30023341. doi:10.3389/fonc.2018.00260. （原始内容存档于2019-12-26）.
^ Andrei, L; Kasas, S; Ochoa Garrido, I; Stanković, T; Suárez Korsnes, M; Vaclavikova, R; Assaraf, YG; Pešić, M. Advanced technological tools to study multidrug resistance in cancer.. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy. 2019-10-17, 48: 100658 [2019-12-26]. PMID 31678863. doi:10.1016/j.drup.2019.100658. （原始内容存档于2019-12-26）.
^ Thomas, LH; Friedland, JS; Sharland, M; Becker, S. Respiratory syncytial virus-induced RANTES production from human bronchial epithelial cells is dependent on nuclear factor-kappa B nuclear binding and is inhibited by adenovirus-mediated expression of inhibitor of kappa B alpha.. Journal of immunology (Baltimore, Md. : 1950). 1998-07-15, 161 (2): 1007–16 [2019-12-26]. PMID 9670982. （原始内容存档于2019-12-26）.
^ Lin, Y; Zhang, M; Barnes, PF. Chemokine production by a human alveolar epithelial cell line in response to Mycobacterium tuberculosis.. Infection and immunity. 1998-03, 66 (3): 1121–6 [2019-12-26]. PMID 9488404. （原始内容存档于2019-12-26）.

外部链接

Cellosaurus entry for A431（页面存档备份，存于互联网档案馆）

[a-1] 1.0 ^1.1 A549 Cell Line: Human alveolar adenocarcinoma cell line -General Information. [2019-12-03]. （原始内容存档于2012-01-05）（英语）.

[2] Xia, H; Yu, C; Zhang, W; Zhang, B; Zhao, Y; Fang, F. [Identification and isolation of cancer stem cells from A549 cells].. Zhongguo fei ai za zhi = Chinese journal of lung cancer. 2013-08-20, 16 (8): 400–404 [2019-12-03]. PMID 23945242. doi:10.3779/j.issn.1009-3419.2013.08.02.

[3] Giard, DJ; Aaronson, SA; Todaro, GJ; Arnstein, P; Kersey, JH; Dosik, H; Parks, WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.. Journal of the National Cancer Institute. 1973-11, 51 (5): 1417–23 [2019-12-03]. PMID 4357758. doi:10.1093/jnci/51.5.1417.

[4] Foster, KA; Oster, CG; Mayer, MM; Avery, ML; Audus, KL. Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism.. Experimental cell research. 1998-09-15, 243 (2): 359–66 [2019-12-03]. PMID 9743595. doi:10.1006/excr.1998.4172.

[5] Korsnes, MS; Korsnes, R. Single-Cell Tracking of A549 Lung Cancer Cells Exposed to a Marine Toxin Reveals Correlations in Pedigree Tree Profiles.. Frontiers in oncology. 2018, 8: 260 [2019-12-26]. PMID 30023341. doi:10.3389/fonc.2018.00260. （原始内容存档于2019-12-26）.

[6] Andrei, L; Kasas, S; Ochoa Garrido, I; Stanković, T; Suárez Korsnes, M; Vaclavikova, R; Assaraf, YG; Pešić, M. Advanced technological tools to study multidrug resistance in cancer.. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy. 2019-10-17, 48: 100658 [2019-12-26]. PMID 31678863. doi:10.1016/j.drup.2019.100658. （原始内容存档于2019-12-26）.

[7] Thomas, LH; Friedland, JS; Sharland, M; Becker, S. Respiratory syncytial virus-induced RANTES production from human bronchial epithelial cells is dependent on nuclear factor-kappa B nuclear binding and is inhibited by adenovirus-mediated expression of inhibitor of kappa B alpha.. Journal of immunology (Baltimore, Md. : 1950). 1998-07-15, 161 (2): 1007–16 [2019-12-26]. PMID 9670982. （原始内容存档于2019-12-26）.

[8] Lin, Y; Zhang, M; Barnes, PF. Chemokine production by a human alveolar epithelial cell line in response to Mycobacterium tuberculosis.. Infection and immunity. 1998-03, 66 (3): 1121–6 [2019-12-26]. PMID 9488404. （原始内容存档于2019-12-26）.

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